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Tuoro sul Trasimeno is a comune (municipality) in the Province of Perugia in the Italian region Umbria, located about 25 km northwest of Perugia.
Tuoro sul Trasimeno borders the following municipalities: Castiglione del Lago, Cortona, Lisciano Niccone, Magione, Passignano sul Trasimeno. It is located on the northern shore of the Lake Trasimeno.